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Image Search Results
Journal: Scientific Reports
Article Title: TRPM2 regulates TXNIP-mediated NLRP3 inflammasome activation via interaction with p47 phox under high glucose in human monocytic cells
doi: 10.1038/srep35016
Figure Lengend Snippet: ( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, ASC- or caspase-1-siRNA, or ( D ) GAPDH- or TRPM2-siRNAs under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.
Article Snippet: Antibodies used for immunoblotting or immunostaining were as follows: anti-rabbit caspase-1 (2225 S, Cell Signaling, US), anti-rabbit IL-1β (sc-7884, Santa Cruz Biotechnology, US), anti-rabbit TRPM2 (LS-C160232, LifeSpan BioSciences, US), anti-goat TRPM2 (LS-B4122, LifeSpan BioSciences, US), anti-rabbit NLRP3 (15101 S, Cell Signaling, US),
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot
Journal: Scientific Reports
Article Title: TRPM2 regulates TXNIP-mediated NLRP3 inflammasome activation via interaction with p47 phox under high glucose in human monocytic cells
doi: 10.1038/srep35016
Figure Lengend Snippet: ( A ) Fluorescence images showing fluo-4-loaded cells taken at indicated timepoints, and representative flow cytometric plot and graph showing relative median fluorescence intensity (MFI) of intracellular Ca 2+ concentration by Fluo-4 staining under low glucose (LG; 5.5 mM glucose;) or high glucose (HG; 30 mM glucose) (n = 5). ( B ) Relative changes in [Ca 2+ ] i , evoked by H 2 O 2 (1 mM) over the time course. The cells were treated in the presence of 2-aminoethoxydiphenyl borate (2-APB; 100 μM), or with pre-treatment of 3-aminobenzamide (3-AB; 5 mM) or 3,4-dihydro-5-[4-(1-piperidinyl)butoxy]-1(2H)-isoquinolinone (DPQ; 100 μM), or GAPDH- (GA si) or TRPM2-siRNAs (TRPM2 si) under HG (n = 4). ( C ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), and GAPDH in the presence of EGTA-AM (0.5, 1, 5 mM) under HG (n = 4). ( D ) Immunofluorescence images showing the location of caspase-1 and ASC in fixed cells using confocal microscopy in the presence of GAPDH- or TRPM2-siRNAs, or EGTA-AM (5 mM) under HG. The percentage of co-localization of caspase-1 with ASC was calculated as the average volume of the overlapping areas (n = 4). Data were shown as mean ± S.E.M. ( A ) *P < 0.05 vs. LG. ( B ) *P < 0.05 and **P < 0.01 vs. HG; ## P < 0.01 vs. HG + GA si. ( D ) **P < 0.01 vs. LG + GA si; ## P < 0.01 vs. HG + GA si.
Article Snippet: Antibodies used for immunoblotting or immunostaining were as follows: anti-rabbit caspase-1 (2225 S, Cell Signaling, US), anti-rabbit IL-1β (sc-7884, Santa Cruz Biotechnology, US), anti-rabbit TRPM2 (LS-C160232, LifeSpan BioSciences, US), anti-goat TRPM2 (LS-B4122, LifeSpan BioSciences, US), anti-rabbit NLRP3 (15101 S, Cell Signaling, US),
Techniques: Fluorescence, Concentration Assay, Staining, Western Blot, Immunofluorescence, Confocal Microscopy