mouse anti asc Search Results


90
Genentech inc rat anti–mouse asc clone 8e4
Rat Anti–Mouse Asc Clone 8e4, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti–mouse asc clone 8e4/product/Genentech inc
Average 90 stars, based on 1 article reviews
rat anti–mouse asc clone 8e4 - by Bioz Stars, 2026-03
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90
Merck KGaA mouse anti-human asc
Mouse Anti Human Asc, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-human asc/product/Merck KGaA
Average 90 stars, based on 1 article reviews
mouse anti-human asc - by Bioz Stars, 2026-03
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90
Genentech inc rat anti-asc
Rat Anti Asc, supplied by Genentech inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rat anti-asc/product/Genentech inc
Average 90 stars, based on 1 article reviews
rat anti-asc - by Bioz Stars, 2026-03
90/100 stars
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90
Absolute Biotech Inc rabbit anti-mouse asc
Rabbit Anti Mouse Asc, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-mouse asc/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
rabbit anti-mouse asc - by Bioz Stars, 2026-03
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90
Merck KGaA anti-asc antibody mouse 04–147
Anti Asc Antibody Mouse 04–147, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-asc antibody mouse 04–147/product/Merck KGaA
Average 90 stars, based on 1 article reviews
anti-asc antibody mouse 04–147 - by Bioz Stars, 2026-03
90/100 stars
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90
Absolute Biotech Inc anti-mouse asc
( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, <t>ASC-</t> or caspase-1-siRNA, or ( D ) GAPDH- <t>or</t> <t>TRPM2-siRNAs</t> under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.
Anti Mouse Asc, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-mouse asc/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
anti-mouse asc - by Bioz Stars, 2026-03
90/100 stars
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90
Trans Genic inc rabbit anti-mouse asc
( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, <t>ASC-</t> or caspase-1-siRNA, or ( D ) GAPDH- <t>or</t> <t>TRPM2-siRNAs</t> under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.
Rabbit Anti Mouse Asc, supplied by Trans Genic inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-mouse asc/product/Trans Genic inc
Average 90 stars, based on 1 article reviews
rabbit anti-mouse asc - by Bioz Stars, 2026-03
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90
Biomol GmbH rabbit polyclonal antibody against human asc-1 (peptide cppsllpatdkpskpq)
( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, <t>ASC-</t> or caspase-1-siRNA, or ( D ) GAPDH- <t>or</t> <t>TRPM2-siRNAs</t> under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.
Rabbit Polyclonal Antibody Against Human Asc 1 (Peptide Cppsllpatdkpskpq), supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibody against human asc-1 (peptide cppsllpatdkpskpq)/product/Biomol GmbH
Average 90 stars, based on 1 article reviews
rabbit polyclonal antibody against human asc-1 (peptide cppsllpatdkpskpq) - by Bioz Stars, 2026-03
90/100 stars
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90
Abnova mouse anti-asc
( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, <t>ASC-</t> or caspase-1-siRNA, or ( D ) GAPDH- <t>or</t> <t>TRPM2-siRNAs</t> under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.
Mouse Anti Asc, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-asc/product/Abnova
Average 90 stars, based on 1 article reviews
mouse anti-asc - by Bioz Stars, 2026-03
90/100 stars
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90
Adipogen anti-mouse asc sc-51441
( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, <t>ASC-</t> or caspase-1-siRNA, or ( D ) GAPDH- <t>or</t> <t>TRPM2-siRNAs</t> under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.
Anti Mouse Asc Sc 51441, supplied by Adipogen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-mouse asc sc-51441/product/Adipogen
Average 90 stars, based on 1 article reviews
anti-mouse asc sc-51441 - by Bioz Stars, 2026-03
90/100 stars
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90
ABclonal Biotechnology rabbit anti-mouse asc
( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, <t>ASC-</t> or caspase-1-siRNA, or ( D ) GAPDH- <t>or</t> <t>TRPM2-siRNAs</t> under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.
Rabbit Anti Mouse Asc, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti-mouse asc/product/ABclonal Biotechnology
Average 90 stars, based on 1 article reviews
rabbit anti-mouse asc - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, ASC- or caspase-1-siRNA, or ( D ) GAPDH- or TRPM2-siRNAs under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.

Journal: Scientific Reports

Article Title: TRPM2 regulates TXNIP-mediated NLRP3 inflammasome activation via interaction with p47 phox under high glucose in human monocytic cells

doi: 10.1038/srep35016

Figure Lengend Snippet: ( A,C ) ELISA for IL-1β or IL-18 secretion from the supernatants of treated cells. ( A ) U937 cells were stimulated with low glucose (LG; 5.5 mM glucose), mannitol (Ma; 30 mM), high glucose (HG; 10, 20, 30 mM glucose) for 48 h (n = 6–7). ( B,D ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), GAPDH and β-actin in the presence of ( B ) GAPDH-, NLRP3-, ASC- or caspase-1-siRNA, or ( D ) GAPDH- or TRPM2-siRNAs under HG. ( C ) U937 or THP-1 cells were stimulated with HG in the presence of GAPDH- or TRPM2-siRNAs, or 3-AB (5 mM) or DPQ (100 μM) (n = 4). Data shown were shown as mean ± S.E.M. ( A,C ) *P < 0.05 , **P < 0.01 and ***P < 0.001 vs. LG; # P < 0.05 and ## P < 0.01 vs. HG.

Article Snippet: Antibodies used for immunoblotting or immunostaining were as follows: anti-rabbit caspase-1 (2225 S, Cell Signaling, US), anti-rabbit IL-1β (sc-7884, Santa Cruz Biotechnology, US), anti-rabbit TRPM2 (LS-C160232, LifeSpan BioSciences, US), anti-goat TRPM2 (LS-B4122, LifeSpan BioSciences, US), anti-rabbit NLRP3 (15101 S, Cell Signaling, US), anti-mouse ASC (LS-C175123, LifeSpan BioSciences, US), anti-rabbit PKC-α (2056 S, Cell Signaling, US), anti-rabbit PKC-α (phospho T497) (ab76016, Abcam, US), anti-rabbit ERK1/2 (9102 S, Cell Signaling, US), anti-rabbit ERK1/2 (Thr202/Tyr204) (9101 S, Cell Signaling, US), anti-goat p22 phox (sc-11712, Santa Cruz Biotechnology, US), anti-rabbit gp91-phox (sc-20782, Santa Cruz Biotechnology, US), anti-rabbit p47 phox (4312 S, Cell Signaling, US), anti-rabbit p47 phox (phospho Ser345) (PA5-37806, Pierce, US), anti-mouse TXNIP (K0204-3, MBL Life science, Japan), anti-rabbit GAPDH (2118 S, Cell Signaling, US), anti-rabbit β-Actin (4967 S, Cell Signaling, US), goat anti-rabbit IgG-HRP (7074 S, Cell signaling, US), donkey anti-goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135, Abcam, US), anti-rabbit IgG (H + L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) (4412 S, Cell Signaling, US).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

( A ) Fluorescence images showing fluo-4-loaded cells taken at indicated timepoints, and representative flow cytometric plot and graph showing relative median fluorescence intensity (MFI) of intracellular Ca 2+ concentration by Fluo-4 staining under low glucose (LG; 5.5 mM glucose;) or high glucose (HG; 30 mM glucose) (n = 5). ( B ) Relative changes in [Ca 2+ ] i , evoked by H 2 O 2 (1 mM) over the time course. The cells were treated in the presence of 2-aminoethoxydiphenyl borate (2-APB; 100 μM), or with pre-treatment of 3-aminobenzamide (3-AB; 5 mM) or 3,4-dihydro-5-[4-(1-piperidinyl)butoxy]-1(2H)-isoquinolinone (DPQ; 100 μM), or GAPDH- (GA si) or TRPM2-siRNAs (TRPM2 si) under HG (n = 4). ( C ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), and GAPDH in the presence of EGTA-AM (0.5, 1, 5 mM) under HG (n = 4). ( D ) Immunofluorescence images showing the location of caspase-1 and ASC in fixed cells using confocal microscopy in the presence of GAPDH- or TRPM2-siRNAs, or EGTA-AM (5 mM) under HG. The percentage of co-localization of caspase-1 with ASC was calculated as the average volume of the overlapping areas (n = 4). Data were shown as mean ± S.E.M. ( A ) *P < 0.05 vs. LG. ( B ) *P < 0.05 and **P < 0.01 vs. HG; ## P < 0.01 vs. HG + GA si. ( D ) **P < 0.01 vs. LG + GA si; ## P < 0.01 vs. HG + GA si.

Journal: Scientific Reports

Article Title: TRPM2 regulates TXNIP-mediated NLRP3 inflammasome activation via interaction with p47 phox under high glucose in human monocytic cells

doi: 10.1038/srep35016

Figure Lengend Snippet: ( A ) Fluorescence images showing fluo-4-loaded cells taken at indicated timepoints, and representative flow cytometric plot and graph showing relative median fluorescence intensity (MFI) of intracellular Ca 2+ concentration by Fluo-4 staining under low glucose (LG; 5.5 mM glucose;) or high glucose (HG; 30 mM glucose) (n = 5). ( B ) Relative changes in [Ca 2+ ] i , evoked by H 2 O 2 (1 mM) over the time course. The cells were treated in the presence of 2-aminoethoxydiphenyl borate (2-APB; 100 μM), or with pre-treatment of 3-aminobenzamide (3-AB; 5 mM) or 3,4-dihydro-5-[4-(1-piperidinyl)butoxy]-1(2H)-isoquinolinone (DPQ; 100 μM), or GAPDH- (GA si) or TRPM2-siRNAs (TRPM2 si) under HG (n = 4). ( C ) Representative immunoblots for pro-IL-1β, IL-1β p17, pro-caspase-1, cleaved caspase-1 (p20), and GAPDH in the presence of EGTA-AM (0.5, 1, 5 mM) under HG (n = 4). ( D ) Immunofluorescence images showing the location of caspase-1 and ASC in fixed cells using confocal microscopy in the presence of GAPDH- or TRPM2-siRNAs, or EGTA-AM (5 mM) under HG. The percentage of co-localization of caspase-1 with ASC was calculated as the average volume of the overlapping areas (n = 4). Data were shown as mean ± S.E.M. ( A ) *P < 0.05 vs. LG. ( B ) *P < 0.05 and **P < 0.01 vs. HG; ## P < 0.01 vs. HG + GA si. ( D ) **P < 0.01 vs. LG + GA si; ## P < 0.01 vs. HG + GA si.

Article Snippet: Antibodies used for immunoblotting or immunostaining were as follows: anti-rabbit caspase-1 (2225 S, Cell Signaling, US), anti-rabbit IL-1β (sc-7884, Santa Cruz Biotechnology, US), anti-rabbit TRPM2 (LS-C160232, LifeSpan BioSciences, US), anti-goat TRPM2 (LS-B4122, LifeSpan BioSciences, US), anti-rabbit NLRP3 (15101 S, Cell Signaling, US), anti-mouse ASC (LS-C175123, LifeSpan BioSciences, US), anti-rabbit PKC-α (2056 S, Cell Signaling, US), anti-rabbit PKC-α (phospho T497) (ab76016, Abcam, US), anti-rabbit ERK1/2 (9102 S, Cell Signaling, US), anti-rabbit ERK1/2 (Thr202/Tyr204) (9101 S, Cell Signaling, US), anti-goat p22 phox (sc-11712, Santa Cruz Biotechnology, US), anti-rabbit gp91-phox (sc-20782, Santa Cruz Biotechnology, US), anti-rabbit p47 phox (4312 S, Cell Signaling, US), anti-rabbit p47 phox (phospho Ser345) (PA5-37806, Pierce, US), anti-mouse TXNIP (K0204-3, MBL Life science, Japan), anti-rabbit GAPDH (2118 S, Cell Signaling, US), anti-rabbit β-Actin (4967 S, Cell Signaling, US), goat anti-rabbit IgG-HRP (7074 S, Cell signaling, US), donkey anti-goat IgG H&L (Alexa Fluor® 647) preadsorbed (ab150135, Abcam, US), anti-rabbit IgG (H + L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) (4412 S, Cell Signaling, US).

Techniques: Fluorescence, Concentration Assay, Staining, Western Blot, Immunofluorescence, Confocal Microscopy